C-terminal hemagglutinin tag

Author: pyyz

August undefined, 2024

WebThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. ... The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. Application References (PubMed link indicates BioLegend ... WebC Terminal Hemagglutinin Ha Epitope Tag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - …

Hemagglutinin A Ha Tag ATCC Bioz

http://www.lianshimall.com/goods-2805139.html WebSep 28, 2024 · Activation of GPR133 signaling with antibodies against its N-terminus To test whether GPR133 signaling is modulated by antibodies binding to the extracellular N-terminus, we transfected HEK293T cells with GPR133 tagged with an N-terminal hemagglutinin (HA) and a C-terminal FLAG epitope (Fig. 1A). chinese restaurants hayward wi

C Terminal Ha Tag Addgene inc Bioz

WebSep 13, 2024 · the C-terminal epitope of GPR126 no change in cAMP levels was observed (Supp. Fig. S1A). ... In our receptor constructs, we inserted an N-terminal hemagglutinin tag (HA-TAG, red pentagon) immediately distal to the signal peptide and a C-terminal Flag tag (FLAG-TAG, yellow pentagon) right before the Stop codon. WebThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA. Product Details Technical data sheet Product Details WebHA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. chinese restaurants hayward ca

Plasmodium falciparum Erythrocytic Stage Parasites Require the …

WebAug 5, 2024 · These studies used strains in which SGS1 was C-terminally tagged with three or six copies of a human influenza hemagglutinin-derived epitope tag (3HA and 6HA). … WebAug 5, 2012 · Western blotting using an antibody against the C-terminal hemagglutinin (HA) tag of the reporter (anti-HA) or against GFP (anti-GFP) detected the 60-kDa GFP-mCherry-HA fusion protein in the... chinese restaurant shelleyWebThe antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This … chinese restaurant shelford

"WebC-terminal hemagglutinin tag) (21). Cultures were preincubated with STI571 or the -secretase inhibitor L-685,458 for 2 h, then pulse labeled for 30 min with [35S]methionine [35S]cysteine Easy Tag Protein Labeling Mix (Perkin–Elmer) and chased for 2 h with inhibitors present. Media were immunoprecipitated with " - C-terminal hemagglutinin tag

C-terminal hemagglutinin tag

National Center for Biotechnology Information

WebThe affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some … WebThese mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-IP studies but are also easily detected via Western blot.

Did you know?

WebThe mature HA1/HA2 complex contains a membrane-proximal stalk domain containing the N- and C-terminal portions of HA1 and the entirety of HA2 and a membrane-distal receptor-binding domain containing receptor-binding and vestigial esterase subdomains (Figure 3B).

WebAn HA-tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope … WebThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is …

WebJun 25, 2013 · This suggests a post-translational processing of PfAtg7. We are able to attenuate levels of endogenous PfAtg7 through integration of a C-terminal regulatable fluorescent affinity (RFA) tag that allows for rapid destabilizion of … WebMar 25, 2024 · Furthermore, HPIV3F-Flag, which is a recombinant HPIV3 with a Flag tag at the C-terminus of the F protein, was constructed and recovered. We found that the M, F, and hemagglutinin-neuraminidase (HN) proteins and the viral genome can accumulate in lipid rafts in HPIV3F-Flag-infected cells, and the F protein mainly exists in the form of F1 …

WebBiotinylated Influenza A [A/Hong Kong/483/97(H5N1)] Hemagglutinin 1 (HA1) Protein, His Tag,Avitag™ (MALS verified) 400-682-2521 [email protected] 注册登录登录注册关注公众号

WebAug 26, 2024 · The overexpressed GFP-tagged NT proteins were produced at an elevated level relative to the endogenous full-length protein (hemagglutinin (HA)-tagged) (Figure 1B,C). Truncation of the first 11 amino acids (NT12) appeared to slightly increase the steady state level of the expressed protein (albeit, not statistically significant), but further ... chinese restaurants hazel park miWebIdentification of the C- Terminal Residue. The C-terminal residue is determined by the use of either a chemical reagent or the enzyme carboxypeptidase. The chemical reagent … grand team trading groupWebThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is … grand tea co golden flowerHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA-tag is derived from the HA-molecule corresponding to amino acids 98-106. HA-tag has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins … See more The DNA sequences for the HA-tag include: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The resulting amino acid sequence: YPYDVPDYA (Tyr … See more • Biology portal • Protein tag • SpyTag See more • Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (May 1988). "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method". Molecular and Cellular Biology. 8 … See more chinese restaurants hazel dell waWebAbcam c terminal hemagglutinin ha tag C Terminal Hemagglutinin Ha Tag, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more chinese restaurants hempstead txWebTag / Fusion Protein. HA (N terminal on backbone) Growth in Bacteria. Bacterial Resistance(s) Ampicillin, 100 μg/mL Growth Temperature. 37°C ... pCDNA3- c-terminal 6xMyc pcDNA3.1-mCherry RGS-6xHis-pcDNA3.1- … grand team technologies limitedWebOne research group found that more C-terminal fusion proteins localize to the intended subcellular compartment than N-terminally tagged fusion proteins 2. However, it is important to stress that while C-terminally tagged proteins tend to localize and behave as expected, this is not always possible to predict. chinese restaurants heights houston