Indigoidine biosynthesis

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August undefined, 2024

WebIndigoidine is a natural blue pigment produced by several bacteria via known biosynthetic gene clusters. 8–12 This 3′,3′-bipyridyl pigment is formed through condensation of two … Web22 jul. 2024 · The synthetic indigoidine biosynthesis module (Genbank: MW629112) was first inserted into the NdeI and SacI sites of pET-21a to generate pT7lac-ind. …

Indigoidine – Biosynthesized organic semiconductor - ScienceDirect

Web1 dec. 2024 · Indigoidine is a blue natural pigment, which can be efficiently synthetized in E. coli. In addition to its antioxidant and antimicrobial activities indigoidine due to its … WebAmong the reported biosynthetic enzymes, 67 polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) are most commonly 68 observed. The water-insoluble blue pigment indigoidine [5,5'-diamino-4,4'-dihydroxy-3,3'- 69 diazadiphenoguinone-(2,2')] was isolated from phytopathogenic Erwinia[21] and other … meghan trainor the best part interlude

Relevant Enzymes in a Sentence

Web6 dec. 2013 · Soft rot Enterobacteria in the genera Pectobacterium and Dickeya cause rotting of many crop plants. A new Dickeya isolate has been suggested to form a separate species, given the name Dickeya solani. This bacterium is spreading fast and replacing the closely related, but less virulent, potato pathogens. The genome of D. solani isolate D … WebRelevant Enzymes in a Sentence WebKEY POINTS: • Biosynthesis pathway of indigoidine reconstructed in a high copy number plasmid in E. coli. • Visual and colorimetric detection of Hg (II) and Pb (II) by … nanga def meaning wolof to english

Divergent Biosynthesis of C-Nucleoside Minimycin and …

Indigoidine C10H8N4O4 - PubChem

WebConstruction of protein scaffolds related to pigment biosynthesis. In addition to fusion enzymes, the protein scaffolds are often constructed to improve yields of pigments. The highest yield of indigoidine was 12 g/L after construction of protein scaffold, which was two-fold higher than the control without scaffold (Wang et al., 2024). Web23 jun. 2024 · Indigoidine [5,5’-diamino-4,4’-dihydroxy-3,3’-diazadiphenoguinone-(2,2’)] is a water-insoluble dark blue pigment belonging to the pyridine class, which consists of two … meghan trainor the love trainWebIt is also reported to be involved in the synthesis of indigoidine, which is a blue pigment synthesised by Erwinia chrysanthemi implicated in pathogenicity and protection from oxidative stress. IdgA is involved in indigoidine biosynthesis, but its specific function is unknown [ 2] . Add your annotation Contributing Member Database Entr ies Pfam: nanga clinic port moresby

"Web20 dec. 2024 · Although MIN and indigoidine have been known for decades, the logic underlying the divergent biosynthesis of these interesting molecules has been obscure. … " - Indigoidine biosynthesis

Indigoidine biosynthesis

Investigation of Indigoidine Synthetase Reveals a Conserved …

Web#ID = #Block = #Row = #Column = #GB_ACC = #GENE_ID = #SPOT_ID = #SEQUENCE = #Name = #description = #probe = #Archival BGI gene = #BGI gene = #geneid = #gene symbol = #gene description = #mRNA = #protein = #Nucleotide EST = #Nucleotide Core = #protein = #molecular_function = #biological_process = #cellular_component = #KEGG … Web1 jan. 2013 · To reconstitute the biosynthesis of indigoidine, the correct transformant was grown in LB broth supplemented with 50 μg/ml kanamycin at 37 °C and 250 rpm. When …

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WebInterestingly, indigoidine synthetase genes are widely distrib- uted in bacteria, including Actinobacteriaand Proteobacteria (Figure S1), and frequently colocalized with genes encoding enzymes that glycosylate C-5 of 1, leading to the water-soluble blue pigment indochrome20,21, and the C-nucleoside antibiotic, minimycin13(Figure 1b). Web4.1.3 Desaturation of a tethered glutamine in indigoidine biosynthesis. The purple pigment indigoidine is generated as a virulence factor, possibly as an oxygen radical scavenger, by the phytopathogenic bacterium Erwinia chrysanthemi and also other pathogens and saprophytic bacteria such as S. lavendulae. 23–25 The indigoidine chromophore ...

WebDickeya dadantii is a broad host range phytopathogenic bacterium provoking soft rotness condition on many plants including Arabidopsis. We showed that, after D. dadantii infection, the expression of the Arabidopsis BOS1 gene was specifically induced by the furniture of the bacterial PelB/C pectinases able to debase pectin. Those prompted us at analyze the … Web21 jun. 2024 · To quickly assess the route by which indigoidine is biosynthesized, the abilities of IdgS and IdgS-TE* S1102A to reduce FMN to FMNH 2 were monitored under …

Web11 apr. 2024 · Request PDF Investigating and Optimizing the Lysate-Based Expression of Nonribosomal Peptide Synthetases Using a Reporter System Lysate-based cell-free expression (CFE) systems are accessible ... Web10 apr. 2024 · Genetic compensation of triacylglycerol biosynthesis in the green microalga Chlamydomonas reinhardtii. ... Dinoflagellate phosphopantetheinyl transferase (PPTase) and thiolation domain interactions characterized using …

Web24 jun. 2024 · Here, by studying indigoidine synthetase, a single-module NRPS involved in the biosynthesis of indigoidine and several other bacterial secondary metabolites, we …

WebIn this study, IdgS, the biosynthetic enzyme for indigoidine, was dissected by in vivo and in vitro studies. We found that (S)‐3‐ aminopiperidine‐2,6‐dione (2), the pharmacophore of thalidomide, is a key intermediate of indigoidine biosynthesis. A biocatalyst that converts L‐glutamine to (S)‐2 was then developed. meghan trainor then and nowWeb23 feb. 2024 · There are two known natural 5-ALA biosynthesis pathways. One is the C4 pathway found in yeasts, mammals, and some photosynthetic bacteria [ 1 ]. In this pathway, 5-ALA synthetase (ALAS) condenses succinyl-CoA and glycine to directly synthesize 5 … nanga events and consultingWebDue to the solubility limits of indigoidine, different concentrations of DMSO were added to the lysate reaction mix to dissolve indigoidine: A) 0% DMSO, ... Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis. ACS Synth. Biol. 2024, 6 (1), 39–44. nanga cotton down hantenWebSequences: 7819: total: 4595 (59 %) with assignment: 537: average length: 34 % amino acid coverage : Domains: 6642: total: 841: unique superfamilies : 614: unique ... meghan trainor the voice duetWebThe draft genome sequence and annotation of this bacterium together with previously unreported aspects of its phenotype are described, showing that TF-218T possesses all of the genes for indigoidine biosynthesis, and on specific media the strain showed a blue pigmentation. 19 View 1 excerpt, cites background nanga bush camp weddingWebAbstract: The marine roseobacter Phaeobacter sp. strain Y4I synthesizes the blue antimicrobial secondary metabolite indigoidine when grown as biofilms or on agar plates. Prior studies suggested... meghan trainor the voice ukWebIndigoidine is a bacterial natural product with antioxidant and antimicrobial activities. Its bright blue color resembles the industrial dye indigo, thus representing a new natural … meghan trainor this love is bad for me