WebSep 19, 2024 · TCEP-reduced cells showed cell-surface-specific Alexa-488-maleimide labelling with very little fluorescence occurring from the cytoplasm or nucleus (figure 1 d). This showed that Alexa-488-maleimide can be used to selectively label and visualize cell surface thiols. Optimal reduction conditions were 2.5 mM TCEP for 15 min.

Efficient Site-Specific Labeling of Proteins via Cysteines

WebThe Affibody molecules are partially dimerized due to S-S bridges formed by the C-terminal cysteine and must be reduced prior to labeling with IRDye 800CW Maleimide. Prepare a … Webmolar equivalents of TCEP at 30 ̊C for 2 hours to afford different numbers of available sulfhydryl groups on the antibody molecule, then the reduced antibody was then conjugated with various molar equivalents of maleimide-PEG modified to afford a range of different DARs. Condition: a. TCEP at 30 ̊C for 2 hours; b maleimide-drug, PBS, 7.4, EDTA el rey network live streaming

Maleimide - an overview ScienceDirect Topics

WebFrom pH 6.5 to pH 7.5, the thiol-maleimide reaction is chemoselective for thiols. At pH 7.0, the reaction rate of maleimide with thiols is about 1,000 times faster than the reaction rate of maleimide with amines. However, … WebThe ratio of TCEP and maleimide dye to the protein or peptide must be optimized for efficient reduction and labeling. † Use the highest practical concentration of protein or peptide for the labeling reaction. Other things being equal, more concentrated solutions will react faster than less concentrated ones. WebJul 31, 2024 · Recently, we designed an inventive paradigm in nanomedicine—drug-free macromolecular therapeutics (DFMT). The ability of DFMT to induce apoptosis is based on biorecognition at cell surface, and crosslinking of receptors without the participation of low molecular weight drugs. The system is composed of two nanoconjugates: a bispecific … el rey myrtle beach